GETTING MY SR-4835 TO WORK

The cDNA sequence of human NAMPT was amplified by PCR from a pGex-6p-three-hNAMPT plasmid (kindly gifted from Dr Shui-qing YE, College of Missouri). The PCR products ended up digested and cloned right into a pET21a+ vector making use of NdeI and XhoI restriction enzymes. A point mutation was launched by the fast improve site-directed mutagenesis sy